The Importance of Lighting Sources in Tissue Culture

Oct 13, 2025 by wbrum.org 23 views

The Effect of Lighting Sources on the Growth of Micro Barangan Barangan Red (Musa Acuminata Linn.) Using a Temporary Immersion System

Introduction

The growth and development of plants in tissue culture are influenced by various factors, including the type of lighting source used. Lighting sources play a crucial role in providing the necessary light spectrum for photosynthesis, which is essential for plant growth and development. In this study, we aim to explore the effect of lighting sources on the growth of micro buds of the Barangan red banana plant (Musa Acuminata Linn.) using a temporary immersion system (Temporary Immersion System). The study was conducted in the tissue culture laboratory of PT. Hijau Surya Biotechindo, located in Kisaran, Asahan Regency, North Sumatra Province, during the period May to July.

Research Methodology

The parameters observed in this study include the percentage of shoots formation, number of shoots, bud length, number of leaves, the percentage of roots formed, the number of roots, root length, leaf color, and the incidence of necrosis and vitrification. This observation aims to get a deeper understanding of how the difference in lighting sources can affect the growth of micro banana shoots. The method used in this study is a complete random design (RAL) with two factors, namely the light source in the form of LED lamps and fluorescent lamps, each with one replication.

The Importance of Lighting Sources in Tissue Culture

Lighting sources play a crucial role in tissue culture, as they provide the necessary light spectrum for photosynthesis. The type of lighting source used can affect the growth and development of plants in tissue culture. In this study, we aim to explore the effect of lighting sources on the growth of micro buds of the Barangan red banana plant. The results of this study can provide valuable information on the importance of lighting sources in tissue culture and can help to optimize the growth and development of plants in tissue culture.

Research Result

The results of the study show that the length of the shoot is a parameter that is significantly influenced by the lighting source used. In this case, lighting using fluorescent lamps has proven to be the best to support the growth of the length of the Barangan red banana plant. Although there are differences in the length of the bud parameter, other parameters such as the percentage of shoots formation, number of shoots, number of leaves, the percentage of roots formed, the number of roots, roots, leaf color, necrosis, and vitrification do not show significant effects.

The Benefits of Fluorescent Lamps

The use of fluorescent lamps as a source of lighting in this study provides benefits in terms of light spectrum that is more suitable for the process of photosynthesis, compared to LED lights. Fluorescent lamps emit light with a wider spectrum that can increase the rate of buds growth. On the other hand, although LED lights are more efficient and have a longer life, the spectrum of light produced sometimes cannot meet the optimal needs of plant photosynthesis.

The Importance of Selecting Appropriate Lighting Sources

The selection of appropriate lighting sources is very relevant, especially for plants that have special light needs. This study shows that optimal lighting can increase the growth of shoots, which in turn can have a positive impact on productivity and the quality of agricultural products. The results of this study can provide valuable information on the importance of selecting appropriate lighting sources in tissue culture and can help to optimize the growth and development of plants in tissue culture.

Conclusion

Overall, this research confirms the importance of lighting sources in the growth of micro buds of the Barangan red banana plant. The results show that fluorescent lamps give better results compared to LED lights, especially in terms of buds. Further research is needed to explore a combination of various light sources and their effect on other growth parameters, in order to maximize the results of the culture of banana plants and other plants in the future.

Recommendations

Based on the results of this study, we recommend the use of fluorescent lamps as a source of lighting in tissue culture, especially for plants that have special light needs. Additionally, further research is needed to explore a combination of various light sources and their effect on other growth parameters, in order to maximize the results of the culture of banana plants and other plants in the future.

Limitations of the Study

This study has several limitations, including the use of only two types of lighting sources (LED lamps and fluorescent lamps) and the limited number of replications. Further research is needed to explore a combination of various light sources and their effect on other growth parameters, in order to maximize the results of the culture of banana plants and other plants in the future.

Future Research Directions

Future research directions include exploring a combination of various light sources and their effect on other growth parameters, such as the percentage of shoots formation, number of shoots, number of leaves, the percentage of roots formed, the number of roots, roots, leaf color, necrosis, and vitrification. Additionally, further research is needed to explore the effect of lighting sources on the growth and development of other plants in tissue culture.

Conclusion

In conclusion, this study confirms the importance of lighting sources in the growth of micro buds of the Barangan red banana plant. The results show that fluorescent lamps give better results compared to LED lights, especially in terms of buds. Further research is needed to explore a combination of various light sources and their effect on other growth parameters, in order to maximize the results of the culture of banana plants and other plants in the future.
Frequently Asked Questions (FAQs) About the Effect of Lighting Sources on the Growth of Micro Barangan Barangan Red (Musa Acuminata Linn.) Using a Temporary Immersion System

Q: What is the purpose of this study?

A: The purpose of this study is to explore the effect of lighting sources on the growth of micro buds of the Barangan red banana plant (Musa Acuminata Linn.) using a temporary immersion system (Temporary Immersion System).

Q: What are the parameters observed in this study?

A: The parameters observed in this study include the percentage of shoots formation, number of shoots, bud length, number of leaves, the percentage of roots formed, the number of roots, root length, leaf color, and the incidence of necrosis and vitrification.

Q: What are the results of this study?

A: The results of this study show that the length of the shoot is a parameter that is significantly influenced by the lighting source used. In this case, lighting using fluorescent lamps has proven to be the best to support the growth of the length of the Barangan red banana plant.

Q: What are the benefits of using fluorescent lamps?

A: The use of fluorescent lamps as a source of lighting in this study provides benefits in terms of light spectrum that is more suitable for the process of photosynthesis, compared to LED lights. Fluorescent lamps emit light with a wider spectrum that can increase the rate of buds growth.

Q: What are the limitations of this study?

A: This study has several limitations, including the use of only two types of lighting sources (LED lamps and fluorescent lamps) and the limited number of replications. Further research is needed to explore a combination of various light sources and their effect on other growth parameters.

Q: What are the future research directions?

A: Future research directions include exploring a combination of various light sources and their effect on other growth parameters, such as the percentage of shoots formation, number of shoots, number of leaves, the percentage of roots formed, the number of roots, roots, leaf color, necrosis, and vitrification.

Q: What are the implications of this study?

A: The results of this study have implications for the optimization of lighting sources in tissue culture, particularly for plants that have special light needs. The study suggests that optimal lighting can increase the growth of shoots, which in turn can have a positive impact on productivity and the quality of agricultural products.

Q: Can this study be applied to other plants?

A: Yes, the results of this study can be applied to other plants that have similar light requirements. However, further research is needed to explore the effect of lighting sources on the growth and development of other plants in tissue culture.

Q: What are the recommendations of this study?

A: Based on the results of this study, we recommend the use of fluorescent lamps as a source of lighting in tissue culture, especially for plants that have special light needs. Additionally, further research is needed to explore a combination of various light sources and their effect on other growth parameters.

Q: What are the future applications of this study?

A: The results of this study can be applied in various fields, including agriculture, horticulture, and biotechnology. The study can help to optimize the growth and development of plants in tissue culture, which can lead to improved productivity and quality of agricultural products.

Q: Can this study be replicated?

A: Yes, this study can be replicated with modifications to explore the effect of lighting sources on the growth and development of other plants in tissue culture.