The Importance of Growth Regulatory Hormones in Tissue Culture

Oct 13, 2025 by wbrum.org 20 views

Growth Response of Sipahutar Pineapple Tunas Culture (Ananas comosus (L.) Merr) on MS media with a combination of 2,4-D and BAP

Introduction

The cultivation of Sipahutar pineapple (Ananas comosus (L.) Merr) has been a significant focus in the field of horticulture, particularly in North Sumatra, Indonesia. The demand for this fruit is increasing, and the need for efficient and sustainable cultivation methods has become a pressing concern. One of the most promising approaches to address this challenge is through the use of tissue culture techniques. This method allows for the rapid multiplication of plants, resulting in uniform and high-quality crops. However, the success of tissue culture depends on various factors, including the selection of suitable growth regulatory hormones.

The Importance of Growth Regulatory Hormones in Tissue Culture

Growth regulatory hormones play a crucial role in the development and growth of plants. In tissue culture, these hormones are used to stimulate the growth of shoots, roots, and leaves. The two most commonly used hormones in tissue culture are 2,4-D and BAP. 2,4-D is a synthetic auxin that promotes cell division and differentiation, while BAP is a cytokinin that stimulates cell growth and division. The combination of these two hormones has been shown to have a synergistic effect, resulting in increased growth and productivity.

Methodology

This study employed a complete random design (RAL) with a factorial design of two factors. The researchers tested three levels of concentration of 2,4-D (0 mg/l, 0.5 mg/l, and 1 mg/l) and four levels of BAP concentration (0 mg/l, 1 mg/l, 2 mg/l, and 3 mg/L). The experiment was conducted at the Department of Food Crops and Horticulture, Medan, North Sumatra, from September 2019 to January 2020.

Results and Discussion

The results of the statistical analysis showed that the combination of BAP and 2,4-D had a significant effect on the time of emergence of shoots, the number of shoots produced, the number of leaves formed, and the wet weight of culture. The most effective combination was found at a concentration of 2.4-D 1 mg/L and BAP 3 mg/L (D2B3), which produced the highest number of shoots (15 shoots), the number of leaves reached 35 strands, and the highest wet culture weight was 2.37 grams.

This finding highlights the importance of selecting the right hormone concentration in tissue culture. The use of the two hormones simultaneously can significantly increase the growth of Sipahutar pineapple shoots. The results of this study also underscore the need for a deep understanding of the interaction between plant hormones to achieve optimal results.

Conclusion and Recommendations

This study demonstrates the potential of tissue culture techniques in increasing plant production, particularly in horticultural commodities such as pineapple. The selection of the right hormone concentration can optimize the time and amount of harvest. The combination of 2,4-D and BAP has been successfully tested and can be used as a reference for farmers and researchers in an effort to increase agricultural productivity, especially in the cultivation of Sipahutar pineapple.

In the context of modern agriculture, the use of tissue culture techniques not only increases the quantity of agricultural products but can also accelerate the restoration of plants from disease attacks. Further development in this field may bring up the superior variety of Sipahutar pineapple that is more resistant to pests and diseases, which in turn will provide greater economic benefits for farmers.

Future Directions

By continuing to innovate and apply modern agricultural technology, we can ensure the sustainability of food production and meet the increasing market needs. This study made a significant contribution to the development of tissue culture techniques and could be a model for similar research in the future. The findings of this study can be used as a reference for further research on the optimization of hormone concentrations and the development of new tissue culture techniques.

Additional Analysis and Explanation

This study underlined the importance of tissue culture techniques in increasing plant production, especially in horticultural commodities such as pineapple. With the selection of the right hormone concentration, researchers can optimize the time and amount of harvest. The hormones 2,4-D and BAP have a key role in stimulating the growth of shoots; 2,4-D functions in the cell dedication process, while BAP increases the proliferation of shoots.

From the results of this study, it can be concluded that a deep understanding of the interaction between plant hormones is very important to achieve optimal results. The combination that has been successfully tested can be used as a reference for farmers and researchers in an effort to increase agricultural productivity, especially in the cultivation of Sipahutar pineapple.

Conclusion

By continuing to innovate and apply modern agricultural technology, we can ensure the sustainability of food production and meet the increasing market needs. This study made a significant contribution to the development of tissue culture techniques and could be a model for similar research in the future.
Frequently Asked Questions (FAQs) about the Growth Response of Sipahutar Pineapple Tunas Culture (Ananas comosus (L.) Merr) on MS media with a combination of 2,4-D and BAP

Q: What is the significance of this study?

A: This study aims to develop tissue culture techniques for Sipahutar pineapple (Ananas comosus (L.) Merr) to increase plant production, particularly in horticultural commodities. The study focuses on the use of growth regulatory hormones, 2,4-D and BAP, to stimulate the growth of shoots and roots.

Q: What are the main findings of this study?

A: The study found that the combination of 2,4-D and BAP has a significant effect on the time of emergence of shoots, the number of shoots produced, the number of leaves formed, and the wet weight of culture. The most effective combination was found at a concentration of 2.4-D 1 mg/L and BAP 3 mg/L (D2B3), which produced the highest number of shoots (15 shoots), the number of leaves reached 35 strands, and the highest wet culture weight was 2.37 grams.

Q: What are the implications of this study?

A: The findings of this study have significant implications for the cultivation of Sipahutar pineapple. The use of tissue culture techniques can increase plant production, particularly in horticultural commodities. The selection of the right hormone concentration can optimize the time and amount of harvest. The combination of 2,4-D and BAP has been successfully tested and can be used as a reference for farmers and researchers in an effort to increase agricultural productivity.

Q: What are the limitations of this study?

A: This study has several limitations. The experiment was conducted in a controlled environment, and the results may not be applicable to field conditions. Additionally, the study focused on a specific combination of hormones and may not be applicable to other combinations.

Q: What are the future directions of this research?

A: Future research should focus on optimizing the hormone concentrations and developing new tissue culture techniques. Additionally, the study should be replicated in field conditions to validate the results.

Q: How can this study be applied in practice?

A: The findings of this study can be applied in practice by farmers and researchers who are interested in increasing agricultural productivity. The combination of 2,4-D and BAP can be used as a reference for optimizing the time and amount of harvest.

Q: What are the potential benefits of this study?

A: The potential benefits of this study include increased plant production, particularly in horticultural commodities. The use of tissue culture techniques can also accelerate the restoration of plants from disease attacks. Additionally, the study may lead to the development of superior varieties of Sipahutar pineapple that are more resistant to pests and diseases.

Q: What are the potential challenges of this study?

A: The potential challenges of this study include the high cost of equipment and materials, the need for specialized skills and knowledge, and the potential for contamination and disease.

Q: How can this study be replicated?

A: This study can be replicated by following the same experimental design and procedures. The study should be conducted in a controlled environment, and the results should be validated in field conditions.

Q: What are the potential applications of this study?

A: The potential applications of this study include the development of new tissue culture techniques, the optimization of hormone concentrations, and the creation of superior varieties of Sipahutar pineapple.

Q: What are the potential implications of this study for the agricultural industry?

A: The potential implications of this study for the agricultural industry include increased plant production, improved crop yields, and enhanced agricultural productivity. The study may also lead to the development of new agricultural products and services.

Q: What are the potential implications of this study for the environment?

A: The potential implications of this study for the environment include reduced pesticide and fertilizer use, improved soil health, and enhanced biodiversity. The study may also lead to the development of more sustainable agricultural practices.

Q: What are the potential implications of this study for the economy?

A: The potential implications of this study for the economy include increased agricultural productivity, improved crop yields, and enhanced economic growth. The study may also lead to the creation of new jobs and industries.