The Importance of Gastrointestinal Health

Oct 13, 2025 by wbrum.org 56 views

Characterization of Simplicia and Testing of Antiulcer Activity of Fukoids Compounds Isolated from Brown Seaweed (Sargassum Illicifolium Turner C.Agard) in Male White Mice Induced with Ethanol

Introduction

Gastrointestinal diseases, such as peptic ulcers, dyspepsia, and diarrhea, have become increasingly prevalent in society. Peptic ulcers occur due to an imbalance between aggressive factors, namely stomach acid and pepsin, with defensive factors such as gastric mucosa and bicarbonate. Brown seaweed (Sargassum Illicifolium) is known for its rich compounds that have numerous benefits, including antibacterial activities, antidiabetics, anticoagulants, and others. This study aims to isolate fukoids from brown seaweed, carry out characterization, and test the effectiveness of fukoids in increasing the healing rate of gastric ulcers in male mice that have been induced with ethanol.

The Importance of Gastrointestinal Health

Gastrointestinal health is crucial for overall well-being, and any imbalance in the digestive system can lead to various diseases. Peptic ulcers, in particular, are a significant concern, as they can cause severe pain, bleeding, and even perforation of the stomach or small intestine. The current treatment options for peptic ulcers often involve medications that can have side effects and may not address the underlying cause of the disease. Therefore, there is a need for alternative and more effective treatments.

Brown Seaweed: A Rich Source of Compounds

Brown seaweed, such as Sargassum Illicifolium, is a rich source of compounds that have been shown to have various health benefits. These compounds include fukoids, which are polysaccharides that have been found to have anti-inflammatory, antioxidant, and antimicrobial properties. Fukoids have been isolated from various types of seaweed and have been shown to have potential therapeutic applications.

Isolation and Activity Test Method

The isolation of fukoids from brown seaweed involves a series of steps that begin with drying the seaweed talus. After drying, extraction is performed using 0.1 N HCl solvent for 4 hours. The resulting filtrate is then centrifuged at a speed of 3000 rpm for 10 minutes. This process is repeated with the addition of 96% ethanol in a ratio of 1: 1 to precipitate fukoids. The deposits obtained are dried and crushed to get fukoid and isolated that are ready to be tested.

The fukoidan is then tested on male white mice weighing 100-180 g that have been induced with ethanol to stimulate the formation of ulcers. Fukoids isolates are given in the form of suspension with a dose of 50, 100, and 150 mg/kgBB, with Omeprazol as a positive control and NA-CMC as a negative control. This treatment was given for 6 days, and on the 7th day, the stomach of rats that had been dissected were examined to observe the ulcer formed.

Results and Discussion

The results analysis shows that the fukoid and dose of 100 mg/kgBB provides the best ulcer healing effect with a healing rate of 80.69%, better than comparative drugs. This study indicates that the dose of 100 mg/kgBB is an optimal dose that supports the healing of ulcers in this experimental model. The positive effect of fukoids can be caused by its ability to increase the defensive factor of the stomach and relieve aggression from the factors that cause ulcers.

Mechanism of Action of Fukoids

Fukoidan, as a polysaccharide found in brown seaweed, has the potential to have a different mechanism of action in overcoming ulcers, perhaps through strengthening gastric mucosa or reduction of stomach acid secretion. This finding opens the way for further research to explore the potential of fukoids as therapeutic agents in the treatment of peptic ulcer.

Conclusion

With the results of this study, Fukoidan from Sargassum Illicifolium can be considered as an alternative treatment for gastric disease. Further research will be needed to understand in depth about the mechanism of action of fukoids and potential applications in the therapy of digestive tract diseases.

Future Directions

This study provides a foundation for further research on the potential of fukoids as therapeutic agents in the treatment of peptic ulcer. Future studies can focus on exploring the mechanism of action of fukoids, optimizing the dosage and administration of fukoids, and evaluating the efficacy of fukoids in combination with other treatments. Additionally, studies can be conducted to explore the potential of fukoids in treating other gastrointestinal diseases.

References

[List of references cited in the study]

Limitations

This study has several limitations, including the small sample size and the use of a single type of seaweed. Future studies can address these limitations by increasing the sample size and using multiple types of seaweed.

Conclusion

In conclusion, this study demonstrates the potential of fukoids isolated from brown seaweed (Sargassum Illicifolium) as a treatment for gastric disease. The results of this study provide a foundation for further research on the potential of fukoids as therapeutic agents in the treatment of peptic ulcer.
Frequently Asked Questions (FAQs) about Characterization of Simplicia and Testing of Antiulcer Activity of Fukoids Compounds Isolated from Brown Seaweed (Sargassum Illicifolium Turner C.Agard) in Male White Mice Induced with Ethanol

Q: What is the main objective of this study?

A: The main objective of this study is to isolate fukoids from brown seaweed (Sargassum Illicifolium), carry out characterization, and test the effectiveness of fukoids in increasing the healing rate of gastric ulcers in male mice that have been induced with ethanol.

Q: What is the significance of this study?

A: This study is significant because it provides a new alternative treatment for gastric disease, which is a major public health concern. The results of this study can lead to the development of a new therapeutic agent for the treatment of peptic ulcer.

Q: What are the potential benefits of fukoids as a treatment for gastric disease?

A: The potential benefits of fukoids as a treatment for gastric disease include its ability to increase the defensive factor of the stomach and relieve aggression from the factors that cause ulcers. Fukoids may also have anti-inflammatory, antioxidant, and antimicrobial properties.

Q: How were the fukoids isolated from brown seaweed?

A: The fukoids were isolated from brown seaweed through a series of steps that begin with drying the seaweed talus. After drying, extraction was performed using 0.1 N HCl solvent for 4 hours. The resulting filtrate was then centrifuged at a speed of 3000 rpm for 10 minutes. This process was repeated with the addition of 96% ethanol in a ratio of 1: 1 to precipitate fukoids.

Q: What was the experimental design of this study?

A: The experimental design of this study involved inducing male white mice with ethanol to stimulate the formation of ulcers. The mice were then treated with fukoids isolates in the form of suspension with a dose of 50, 100, and 150 mg/kgBB, with Omeprazol as a positive control and NA-CMC as a negative control. The treatment was given for 6 days, and on the 7th day, the stomach of rats that had been dissected were examined to observe the ulcer formed.

Q: What were the results of this study?

A: The results of this study showed that the fukoid and dose of 100 mg/kgBB provided the best ulcer healing effect with a healing rate of 80.69%, better than comparative drugs.

Q: What are the potential applications of fukoids as a treatment for gastric disease?

A: The potential applications of fukoids as a treatment for gastric disease include its use as a therapeutic agent in the treatment of peptic ulcer. Further research is needed to explore the potential of fukoids in treating other gastrointestinal diseases.

Q: What are the limitations of this study?

A: The limitations of this study include the small sample size and the use of a single type of seaweed. Future studies can address these limitations by increasing the sample size and using multiple types of seaweed.

Q: What are the future directions of this research?

A: The future directions of this research include exploring the mechanism of action of fukoids, optimizing the dosage and administration of fukoids, and evaluating the efficacy of fukoids in combination with other treatments. Additionally, studies can be conducted to explore the potential of fukoids in treating other gastrointestinal diseases.

Q: What are the potential benefits of this research for society?

A: The potential benefits of this research for society include the development of a new therapeutic agent for the treatment of peptic ulcer, which can lead to improved health outcomes and reduced healthcare costs.