Pheretima Posthuma worm test animals

Oct 13, 2025 by wbrum.org 50 views

Antelmitting Activity Test: The Combination of Katuk Leaves Ethanol Extract (Sauropus Androgynus (L.) Merr.) And Ethanol Extract of Guava Leaves (Psidium Guajava L.) Against Pheretima Posthuma

Introduction

Diseases caused by worms are a significant problem in tropical and developing countries, including Indonesia. Katuk leaves (Sauropus Androgynus) and Guava Leaves (Psidium Guajava) are known to have antelmitting activity due to their secondary metabolitic content, such as alkaloids, flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids. Pheretima Posthuma worm test animals are chosen because they have anatomical and physiological characteristics similar to worms that can be found in the human intestine. This study aims to determine the characterization of dried powder, the content of secondary metabolites in dry powder and extracts, as well as the activity of antelmitting from the combination of ethanol extract of katuk leaves and ethanol extract of guava leaves against Pheretima Posthuma.

The Importance of Antelmitting Activity

Antelmitting activity is crucial in the treatment of worm infections, which are often a problem in tropical and developing countries. Katuk leaves and Guava Leaves have been traditionally used in medicine to treat various health problems, including worm infections. However, the effectiveness of these plants in treating worm infections has not been extensively studied. This study aims to investigate the antelmitting activity of the combination of ethanol extract of katuk leaves and ethanol extract of guava leaves against Pheretima Posthuma.

Research Methodology

In this study, the steps taken include the manufacture of katuk leaf extract and guava leaves using the method of maceration with 96% ethanol solvent. Furthermore, the characterization of dry powder is carried out, phreening phytochemicals in dry powder and extracts, as well as testing the activity of antelmitting to Pheretima Posthuma.

Characterization of Dry Powder

The characterization of dry powder from katuk leaves shows a water content of 5.99%, and the water-soluble extract content is 7.29% and soluble in ethanol by 7.90%. Meanwhile, the characterization of dried powder of guava leaves shows 8.65% water content, water-soluble extract 21.49%, and extracts that are soluble in ethanol 20.51%. The content of total ash and not acid soluble ash in both types of leaves is also measured.

Secondary Metabolites

Secondary metabolites detected in dry powder and katuk leaf extract include alkaloids, flavonoids, glycosides, tannins, and steroids. On the other hand, secondary metabolites in dry powder and guava leaf extract include flavonoids, glycosides, saponins, tannins, and steroids.

Antelmitting Activity

The combination of ethanol extract from both leaves shows that the paralysis time and time of death of Pheretima Posthuma are shorter than the use of a single extract.

Conclusion

The results showed that the combination of ethanol extract of katuk leaves and guava leaves had a higher effectiveness in moving and killing Pheretima Posthuma compared to a single extract. These findings provide new insights in the development of herbal therapy that can be used to overcome the problem of worm infections, as well as provide a scientific basis for the use of katuk leaves and guava leaves in traditional medicine.

Implications for Public Health

In the context of public health, especially in areas with high prevalence of worm infections, this study provides an alternative treatment that is natural and potentially safer than the use of chemical drugs. The community and researchers are expected to be able to further develop the use of plant extracts to overcome broader health problems.

Future Directions

This study provides a foundation for further research on the antelmitting activity of katuk leaves and guava leaves. Future studies can investigate the mechanism of action of these plants in treating worm infections, as well as their potential use in combination with other herbal remedies.

Limitations of the Study

This study has several limitations, including the use of a single type of worm test animal and the limited number of samples used in the study. Future studies can address these limitations by using multiple types of worm test animals and increasing the number of samples used in the study.

Conclusion

In conclusion, this study provides evidence of the antelmitting activity of the combination of ethanol extract of katuk leaves and ethanol extract of guava leaves against Pheretima Posthuma. These findings have implications for the development of herbal therapy for worm infections and provide a scientific basis for the use of katuk leaves and guava leaves in traditional medicine.
Q&A: Antelmitting Activity Test - The Combination of Katuk Leaves Ethanol Extract (Sauropus Androgynus (L.) Merr.) And Ethanol Extract of Guava Leaves (Psidium Guajava L.) Against Pheretima Posthuma

Q: What is the purpose of this study?

A: The purpose of this study is to determine the characterization of dried powder, the content of secondary metabolites in dry powder and extracts, as well as the activity of antelmitting from the combination of ethanol extract of katuk leaves and ethanol extract of guava leaves against Pheretima Posthuma.

Q: What are the benefits of using katuk leaves and guava leaves in treating worm infections?

A: Katuk leaves and guava leaves have been traditionally used in medicine to treat various health problems, including worm infections. They have antelmitting activity due to their secondary metabolitic content, such as alkaloids, flavonoids, glycosides, saponins, tannins, and steroids/triterpenoids.

Q: What is the significance of using Pheretima Posthuma as a worm test animal?

A: Pheretima Posthuma worm test animals are chosen because they have anatomical and physiological characteristics similar to worms that can be found in the human intestine.

Q: What are the results of the study?

A: The results showed that the combination of ethanol extract of katuk leaves and guava leaves had a higher effectiveness in moving and killing Pheretima Posthuma compared to a single extract.

Q: What are the implications of this study for public health?

A: In the context of public health, especially in areas with high prevalence of worm infections, this study provides an alternative treatment that is natural and potentially safer than the use of chemical drugs.

Q: What are the limitations of this study?

A: This study has several limitations, including the use of a single type of worm test animal and the limited number of samples used in the study.

Q: What are the future directions of this study?

A: This study provides a foundation for further research on the antelmitting activity of katuk leaves and guava leaves. Future studies can investigate the mechanism of action of these plants in treating worm infections, as well as their potential use in combination with other herbal remedies.

Q: Can katuk leaves and guava leaves be used as a treatment for worm infections in humans?

A: While this study provides evidence of the antelmitting activity of katuk leaves and guava leaves, further research is needed to determine their safety and efficacy in humans.

Q: How can katuk leaves and guava leaves be used in traditional medicine?

A: Katuk leaves and guava leaves can be used in traditional medicine as a treatment for worm infections, as well as for other health problems. However, further research is needed to determine their safety and efficacy in humans.

Q: What are the potential side effects of using katuk leaves and guava leaves in traditional medicine?

A: While katuk leaves and guava leaves are generally considered safe, they may cause side effects such as allergic reactions, digestive problems, or interactions with other medications.

Q: Can katuk leaves and guava leaves be used in combination with other herbal remedies?

A: Yes, katuk leaves and guava leaves can be used in combination with other herbal remedies to treat worm infections and other health problems. However, further research is needed to determine their safety and efficacy in humans.

Q: How can katuk leaves and guava leaves be prepared for use in traditional medicine?

A: Katuk leaves and guava leaves can be prepared for use in traditional medicine by drying, grinding, and mixing with other herbs and spices. However, further research is needed to determine the optimal preparation method and dosage.