Liver histopathology analysis

Oct 13, 2025 by wbrum.org 142 views

Subchronic Toxicity Test of Ethanol Extract Attarasa Bark (Litsea Cubeba Lour.) In Mice: A Comprehensive Analysis

Introduction

The use of plant-based treatments has gained significant attention in recent years due to their potential health benefits and minimal side effects. One such plant is Attarasa (Litsea Cubeba Lour.), a member of the Lauraceae family, which has been used in traditional medicine for various purposes, including antidiarrhea, skin disease treatment, antiseptic, muscle spasms, and has antibacterial and anticancer activity. However, the toxic potential of Attarasa bark ethanol extract remains unknown. This study aims to investigate the subchronic toxicity of Attarasa bark ethanol extract in mice during a 28-day administration period.

Background

Subchronic toxicity testing is a crucial step in evaluating the safety of a substance before it is used as a treatment. This type of testing involves administering a substance to test animals in repeated doses over a period of time, typically 28 days. The goal of subchronic toxicity testing is to detect toxic effects that may appear after the administration of a substance in repeated doses. In this study, we used 40 mice, consisting of 20 male and 20 females, which were divided into five groups. The control group received 0.5% Na CMC, while the treatment group received an ethanol extract of Attarasa bark at doses of 75, 150, 300, and 600 mg/kg BW orally every day for 28 days.

Research Methods

The research methods used in this study were designed to evaluate the toxic potential of Attarasa bark ethanol extract in mice. The study involved the following steps:

Animal selection: 40 mice were used, consisting of 20 male and 20 females.
Grouping: The mice were divided into five groups, with the control group receiving 0.5% Na CMC and the treatment group receiving an ethanol extract of Attarasa bark at doses of 75, 150, 300, and 600 mg/kg BW orally every day for 28 days.
Observations: Clinical symptoms, weight changes, death of mice, and measurement of Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) levels were observed every day.
Liver histopathology analysis: Liver histopathology analysis was carried out to evaluate the effects of Attarasa bark ethanol extract on the liver.
Statistical analysis: All data obtained were analyzed using the ANOVA test with the help of a statistical service solution.

Research Results

The results of this study showed that there were no significant differences in observing clinical symptoms, weight changes, and death in all groups. However, there were significant differences in the measurement of ALT and AST levels, as well as liver histopathological analysis between the control group and the treatment group. This suggests that Attarasa bark ethanol extract has a toxic effect on certain doses.

Conclusion

From the results of this study, it can be concluded that the Attarasa bark ethanol extract has the potential to cause toxic symptoms at a dose of 300 mg/kg BW and 600 mg/kg BW given for 28 days. This discovery can provide important insights in the use of Attarasa extracts for treatment purposes. Although Attarasa has significant benefits in traditional medicine, side effects that may be caused need to be considered, especially when used in high doses. Further research is very necessary to understand the mechanism of its toxicity and to determine the safe dose that can be used in treatment.

Analysis

The discovery that the dose of 300 mg/kg and 600 mg/kg BW causes toxic symptoms can provide important insights in the use of Attarasa extracts for treatment purposes. Although Attarasa has significant benefits in traditional medicine, side effects that may be caused need to be considered, especially when used in high doses. Further research is very necessary to understand the mechanism of its toxicity and to determine the safe dose that can be used in treatment.

Implications

With the development of interest in plant-based treatment, this kind of research is very relevant to ensure the safety and effectiveness of herbal products. The results of this toxicity test are expected to help in developing a safer and effective treatment formula for the community. This study highlights the importance of conducting subchronic toxicity testing before using plant-based treatments in humans.

Future Directions

Further research is necessary to understand the mechanism of toxicity of Attarasa bark ethanol extract and to determine the safe dose that can be used in treatment. This study provides a foundation for future research in this area and highlights the importance of conducting subchronic toxicity testing before using plant-based treatments in humans.

Conclusion

In conclusion, this study provides a comprehensive analysis of the subchronic toxicity of Attarasa bark ethanol extract in mice. The results of this study suggest that Attarasa bark ethanol extract has a toxic effect on certain doses. This discovery can provide important insights in the use of Attarasa extracts for treatment purposes. Further research is necessary to understand the mechanism of its toxicity and to determine the safe dose that can be used in treatment.
Frequently Asked Questions (FAQs) About Subchronic Toxicity Test of Ethanol Extract Attarasa Bark (Litsea Cubeba Lour.) In Mice

Q: What is subchronic toxicity testing?

A: Subchronic toxicity testing is a type of toxicity testing that involves administering a substance to test animals in repeated doses over a period of time, typically 28 days. The goal of subchronic toxicity testing is to detect toxic effects that may appear after the administration of a substance in repeated doses.

Q: What is Attarasa (Litsea Cubeba Lour.)?

A: Attarasa (Litsea Cubeba Lour.) is a plant from the Lauraceae family that has been used in traditional medicine for various purposes, including antidiarrhea, skin disease treatment, antiseptic, muscle spasms, and has antibacterial and anticancer activity.

Q: What was the purpose of this study?

A: The purpose of this study was to investigate the subchronic toxicity of Attarasa bark ethanol extract in mice during a 28-day administration period.

Q: How many mice were used in this study?

A: 40 mice were used in this study, consisting of 20 male and 20 females.

Q: What were the doses of Attarasa bark ethanol extract used in this study?

A: The doses of Attarasa bark ethanol extract used in this study were 75, 150, 300, and 600 mg/kg BW orally every day for 28 days.

Q: What were the observations made in this study?

A: The observations made in this study included clinical symptoms, weight changes, death of mice, and measurement of Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) levels.

Q: What was the result of the liver histopathology analysis?

A: The liver histopathology analysis showed significant differences between the control group and the treatment group, indicating a toxic effect of Attarasa bark ethanol extract on the liver.

Q: What are the implications of this study?

A: The results of this study suggest that Attarasa bark ethanol extract has a toxic effect on certain doses. This discovery can provide important insights in the use of Attarasa extracts for treatment purposes. Further research is necessary to understand the mechanism of its toxicity and to determine the safe dose that can be used in treatment.

Q: What are the future directions of this research?

A: Further research is necessary to understand the mechanism of toxicity of Attarasa bark ethanol extract and to determine the safe dose that can be used in treatment. This study provides a foundation for future research in this area and highlights the importance of conducting subchronic toxicity testing before using plant-based treatments in humans.

Q: Why is subchronic toxicity testing important?

A: Subchronic toxicity testing is important because it helps to detect toxic effects that may appear after the administration of a substance in repeated doses. This type of testing is crucial in ensuring the safety and effectiveness of herbal products.

Q: What are the benefits of using plant-based treatments?

A: Plant-based treatments have several benefits, including minimal side effects and potential health benefits. However, it is essential to conduct subchronic toxicity testing before using plant-based treatments in humans to ensure their safety and effectiveness.

Q: What are the limitations of this study?

A: The limitations of this study include the small sample size and the use of a single dose of Attarasa bark ethanol extract. Further research is necessary to confirm the results of this study and to understand the mechanism of toxicity of Attarasa bark ethanol extract.

Q: What are the future applications of this research?

A: The results of this study can be applied to the development of safer and more effective treatment formulas for the community. This study highlights the importance of conducting subchronic toxicity testing before using plant-based treatments in humans.