immunohistochemical examination using CD 10 antibodies

Oct 13, 2025 by wbrum.org 100 views

CD 10 Immunohistochemical Examination Compared to Conventional Microscopic Examination in Assessing Ovarian Brown Cyst in the Pathology Laboratory of the USU Medan Faculty of Medicine

Introduction

In the medical world, accurate diagnosis enforcement is very important, especially in complicated conditions such as ovarian brown cysts. Ovarian brown cysts, which are often the result of endometriosis, can show symptoms similar to bleeding lutein cysts, thus making clinical diagnosis challenging. Therefore, it is essential to use more accurate methods to distinguish these two types of cysts. One approach that is now beginning to be applied is an immunohistochemical examination using CD 10 antibodies, which aims to increase the sensitivity and specificity of conventional histopathology diagnosis.

Background

Ovarian cysts associated with endometriosis and bloody lutein cysts often show similar appearance, due to the presence of blood products in it. This causes not infrequently a discrepancy between clinical diagnosis and pathology, which can lead to non-optimal treatment. Endometriosis, a condition where tissue similar to the lining of the uterus grows outside the uterus, can cause severe pain, infertility, and other complications. Bleeding lutein cysts, on the other hand, are a type of cyst that forms during ovulation and can cause symptoms similar to endometriosis. This study aims to compare the effectiveness of CD 10 immunohistochemical examination with conventional histopathological methods in distinguishing between endometriosis cysts and bloody lutein cysts.

Research Methods

In this study, an analysis of 44 ovarian cyst preparations was previously diagnosed with conventional histopathology examination using hematoxylin-eosin (H&E) coloring. Of the total, 22 specimens are endometriosis cysts and 22 other specimens are bloody lutein cysts. After that, an immunohistochemical examination with CD 10 antibodies to detect positive expressions in endometrial stroma cells was performed. The CD 10 antibody is a marker that is commonly used to detect endometrial stroma cells, which are a type of cell that is present in endometriosis.

Research Results

The results showed that Immunohistochemical CD 10 examination had a higher sensitivity in detecting ovarian endometriosis cysts, which was 72.27%, and specificity of 77.27%. These results confirm that the immunohistochemical method of CD 10 can provide a better diagnostic picture than conventional histopathology examination. Statistically, the difference between the two methods is significant with the value of P = 0.001. This means that the results of the immunohistochemical examination are highly reliable and can be used as a diagnostic tool to distinguish between endometriosis cysts and bloody lutein cysts.

Conclusion

Immunohistochemical examination CD 10 has proven to be beneficial in increasing the accuracy of the diagnosis of endometriosis cysts and distinguishing them from bloody lutein cysts. With the application of this method, it is expected to reduce the number of diagnosis errors and minimize the risk of over or under treatment in patients. This of course will have a positive impact on clinical management on patients with ovarian cysts.

Implications

The findings of this study have significant implications for the diagnosis and treatment of ovarian cysts. The use of immunohistochemical examination with CD 10 antibodies can provide a more accurate diagnosis of endometriosis cysts, which can lead to more effective treatment and improved patient outcomes. Additionally, the use of this method can reduce the risk of misdiagnosis and unnecessary treatment, which can have a significant impact on patient quality of life.

Future Research Directions

Further research is needed to validate these findings and to deepen our understanding of the biological mechanism behind the ovarian cyst. Additionally, studies are needed to investigate the use of CD 10 immunohistochemical examination in other types of ovarian cysts and to explore the potential applications of this method in other fields of medicine.

Keywords

Brown cysts
Endometrioma
Endometriosis
Bloody lutein cysts
Ovaries
Immunohistochemicals
CD 10
Histopathology

References

[1] American College of Obstetricians and Gynecologists. (2019). Endometriosis.
[2] World Health Organization. (2018). Endometriosis.
[3] National Institute of Child Health and Human Development. (2020). Endometriosis.

Limitations

This study has several limitations. The sample size was relatively small, and the study was conducted in a single institution. Additionally, the study only investigated the use of CD 10 immunohistochemical examination in distinguishing between endometriosis cysts and bloody lutein cysts, and did not explore the use of this method in other types of ovarian cysts. Further research is needed to validate these findings and to deepen our understanding of the biological mechanism behind the ovarian cyst.

Future Studies

Future studies should aim to investigate the use of CD 10 immunohistochemical examination in other types of ovarian cysts and to explore the potential applications of this method in other fields of medicine. Additionally, studies are needed to investigate the sensitivity and specificity of CD 10 immunohistochemical examination in detecting endometriosis cysts and to explore the potential uses of this method in clinical practice.

Conclusion

In conclusion, this study provides new insights in the ovarian cyst diagnostic approach, as well as demonstrating the importance of the use of modern technology in increasing the results of diagnosis in the anatomical pathology laboratory. The findings of this study have significant implications for the diagnosis and treatment of ovarian cysts, and highlight the potential benefits of using CD 10 immunohistochemical examination in clinical practice.
CD 10 Immunohistochemical Examination: Frequently Asked Questions

Q: What is CD 10 immunohistochemical examination?

A: CD 10 immunohistochemical examination is a laboratory test that uses antibodies to detect the presence of CD 10 protein in tissue samples. CD 10 is a marker that is commonly used to detect endometrial stroma cells, which are a type of cell that is present in endometriosis.

Q: What is the purpose of CD 10 immunohistochemical examination?

A: The purpose of CD 10 immunohistochemical examination is to provide a more accurate diagnosis of endometriosis cysts and to distinguish them from bloody lutein cysts. This can help to reduce the number of diagnosis errors and minimize the risk of over or under treatment in patients.

Q: How is CD 10 immunohistochemical examination performed?

A: CD 10 immunohistochemical examination is performed by taking a tissue sample from the patient and then applying antibodies to detect the presence of CD 10 protein. The sample is then examined under a microscope to determine the presence or absence of CD 10 protein.

Q: What are the benefits of CD 10 immunohistochemical examination?

A: The benefits of CD 10 immunohistochemical examination include:

Increased accuracy of diagnosis
Reduced risk of misdiagnosis
Minimized risk of over or under treatment
Improved patient outcomes

Q: What are the limitations of CD 10 immunohistochemical examination?

A: The limitations of CD 10 immunohistochemical examination include:

Relatively small sample size
Study conducted in a single institution
Limited investigation of use in other types of ovarian cysts

Q: Can CD 10 immunohistochemical examination be used in other types of ovarian cysts?

A: While CD 10 immunohistochemical examination has been shown to be effective in distinguishing between endometriosis cysts and bloody lutein cysts, its use in other types of ovarian cysts has not been extensively investigated. Further research is needed to determine its effectiveness in these cases.

Q: How does CD 10 immunohistochemical examination compare to conventional histopathology examination?

A: CD 10 immunohistochemical examination has been shown to be more accurate than conventional histopathology examination in distinguishing between endometriosis cysts and bloody lutein cysts. This is due to the use of antibodies to detect the presence of CD 10 protein, which provides a more specific and sensitive diagnosis.

Q: What are the potential applications of CD 10 immunohistochemical examination in other fields of medicine?

A: The potential applications of CD 10 immunohistochemical examination in other fields of medicine include:

Detection of endometrial cancer
Diagnosis of other types of ovarian cysts
Investigation of the role of CD 10 in other diseases

Q: What are the future directions for research on CD 10 immunohistochemical examination?

A: Future research directions for CD 10 immunohistochemical examination include:

Investigation of its use in other types of ovarian cysts
Exploration of its potential applications in other fields of medicine
Further validation of its effectiveness in distinguishing between endometriosis cysts and bloody lutein cysts

Q: What are the implications of this study for clinical practice?

A: The implications of this study for clinical practice include:

Increased use of CD 10 immunohistochemical examination in the diagnosis of endometriosis cysts
Reduced risk of misdiagnosis and unnecessary treatment
Improved patient outcomes

Q: What are the potential risks and complications associated with CD 10 immunohistochemical examination?

A: The potential risks and complications associated with CD 10 immunohistochemical examination are similar to those associated with other laboratory tests, including:

False positive or false negative results
Allergic reactions to the antibodies used
Infection or other complications associated with the tissue sample collection process

Q: How can patients access CD 10 immunohistochemical examination?

A: Patients can access CD 10 immunohistochemical examination through their healthcare provider. The test is typically performed in a laboratory setting and requires a tissue sample from the patient.

Q: What are the costs associated with CD 10 immunohistochemical examination?

A: The costs associated with CD 10 immunohistochemical examination vary depending on the location and the healthcare provider. The test is typically covered by insurance, but patients should check with their provider to determine the costs associated with the test.