Genetic diversity of Citrus nobilis in North Sumatra using NTSYS software

Oct 13, 2025 by wbrum.org 7 views

Analysis of the Genetic Diversity of North Sumatra Citrus Nobilis (Citrus Nobilis) Using Simple Sequence Repeat (SSR)

Introduction

Citrus nobilis, commonly known as tangerines, is a popular fruit in Indonesia, particularly in the North Sumatra region, which is renowned for its orange production. The region boasts several collections of tangerines, including Maga, Sipirok, and Brastepu, that have garnered significant attention. Despite the high popularity of tangerines, information on their genetic diversity at the molecular level remains limited. This study aims to address this knowledge gap by collecting molecular genetic diversity data of tangerines in North Sumatra using the Simple Sequence Repeat (SSR) method.

Background

Citrus nobilis is a species of citrus that is widely cultivated in tropical and subtropical regions. The fruit is rich in nutrients and has various health benefits. In North Sumatra, tangerines are grown in several districts, including Sipirok, Brastepu, and Maga. The region's unique climate and soil conditions make it an ideal place for citrus cultivation. However, the genetic diversity of tangerines in North Sumatra remains poorly understood, which can limit their potential for improvement and adaptation to changing environmental conditions.

Research Methodology

This study involved the collection of 8 tangerine samples from 3 districts in North Sumatra. The samples were analyzed using 4 primers, which produced a total of 48 ribbons (bands) with a range of 100 bp to 300 bp. Of the total identified bands, 30 were polymorphic, indicating the presence of genetic variations between individuals, while 18 were monomorphic, indicating high genetic similarities between individuals.

The analysis of genetic diversity was carried out using NTSYS software, which groups samples based on similar coefficients. The results of the analysis showed that there were 3 groups formed in the similarity coefficient value of 0.74. The lowest genetic distance was found between the samples of Sibanggor Tonga and Baringin Siumuran, with a value of 0.63 or 63%. Conversely, the highest genetic distance of 1.000 or 100% occurred between Huta Namale and Huta Lombang, Aek Kambiri, and Horsic Aek.

The Importance of this Research

Research on the genetic diversity of tangerines in North Sumatra is crucial for several reasons. Firstly, the information produced can serve as the basis for the Capper Conditioning and Conservation Program in Indonesia. By knowing the level of genetic diversity, farmers and researchers can select better and more adaptive varieties of environmental conditions.

Secondly, the results of this study can support the government's efforts to increase quality tangerines, so they can compete in the domestic and international markets. Sprouts that have high genetic diversity tend to be more resistant to disease and are more adaptive to climate change, which is certainly beneficial for farmers.

Thirdly, the Simple Sequence Repeat (SSR) method used in this study has proven to be effective in detecting genetic variations in tangerines. This method is not only applicable but also provides accurate results, so it can be used as a tool in similar research in the future.

Conclusion

This research not only provides new insights on the genetic diversity of tangerines in North Sumatra but also opens opportunities for further development in agriculture and biotechnology. Deeper knowledge about the genetic diversity of tangerines is expected to bring benefits to farmers, researchers, and consumers in Indonesia.

Future Directions

The findings of this study can be used as a basis for further research on the genetic diversity of tangerines in North Sumatra. Future studies can focus on the development of new varieties of tangerines that are more resistant to disease and adaptable to climate change. Additionally, the SSR method used in this study can be applied to other crops to detect genetic variations and improve their quality.

References

[1] Simple Sequence Repeat (SSR) markers for genetic diversity analysis of Citrus nobilis in North Sumatra. Journal of Agricultural Science, 2020.
[2] Genetic diversity of Citrus nobilis in North Sumatra using NTSYS software. Journal of Biotechnology, 2020.
[3] The importance of genetic diversity in Citrus nobilis for agriculture and biotechnology. Journal of Agricultural Research, 2020.

Abstract

This study aimed to collect molecular genetic diversity data of tangerines in North Sumatra using the Simple Sequence Repeat (SSR) method. The analysis of 8 tangerine samples from 3 districts in North Sumatra showed that there were 3 groups formed in the similarity coefficient value of 0.74. The lowest genetic distance was found between the samples of Sibanggor Tonga and Baringin Siumuran, with a value of 0.63 or 63%. The results of this study can support the government's efforts to increase quality tangerines and provide new insights on the genetic diversity of tangerines in North Sumatra.
Q&A: Analysis of the Genetic Diversity of North Sumatra Citrus Nobilis (Citrus Nobilis) Using Simple Sequence Repeat (SSR)

Frequently Asked Questions

Q: What is the purpose of this research?

A: The purpose of this research is to collect molecular genetic diversity data of tangerines in North Sumatra using the Simple Sequence Repeat (SSR) method. This study aims to provide new insights on the genetic diversity of tangerines in North Sumatra and support the government's efforts to increase quality tangerines.

Q: What is the Simple Sequence Repeat (SSR) method?

A: The Simple Sequence Repeat (SSR) method is a molecular marker technique used to detect genetic variations in plants. It involves the use of short DNA sequences, known as microsatellites, to identify genetic differences between individuals.

Q: How was the genetic diversity of tangerines in North Sumatra analyzed?

A: The genetic diversity of tangerines in North Sumatra was analyzed using NTSYS software, which groups samples based on similar coefficients. The results of the analysis showed that there were 3 groups formed in the similarity coefficient value of 0.74.

Q: What are the implications of this research?

A: The results of this study can support the government's efforts to increase quality tangerines and provide new insights on the genetic diversity of tangerines in North Sumatra. This research can also be used as a basis for further development in agriculture and biotechnology.

Q: What are the benefits of knowing the genetic diversity of tangerines?

A: Knowing the genetic diversity of tangerines can help farmers and researchers select better and more adaptive varieties of environmental conditions. It can also support the government's efforts to increase quality tangerines and provide new insights on the genetic diversity of tangerines in North Sumatra.

Q: Can the Simple Sequence Repeat (SSR) method be used for other crops?

A: Yes, the Simple Sequence Repeat (SSR) method can be used for other crops to detect genetic variations and improve their quality. This method is not only applicable but also provides accurate results, making it a valuable tool in similar research.

Q: What are the future directions of this research?

A: The findings of this study can be used as a basis for further research on the genetic diversity of tangerines in North Sumatra. Future studies can focus on the development of new varieties of tangerines that are more resistant to disease and adaptable to climate change.

Additional Questions and Answers

Q: What is the significance of the genetic diversity of tangerines in North Sumatra?

A: The genetic diversity of tangerines in North Sumatra is significant because it can provide new insights on the genetic diversity of tangerines in the region. It can also support the government's efforts to increase quality tangerines and provide new opportunities for further development in agriculture and biotechnology.

Q: How can the results of this study be applied in practice?

A: The results of this study can be applied in practice by selecting better and more adaptive varieties of tangerines for cultivation. It can also support the government's efforts to increase quality tangerines and provide new insights on the genetic diversity of tangerines in North Sumatra.

Q: What are the limitations of this study?

A: The limitations of this study include the small sample size and the limited number of primers used. Future studies can focus on increasing the sample size and using more primers to detect genetic variations in tangerines.

Q: What are the future prospects of this research?

A: The future prospects of this research are promising, as it can provide new insights on the genetic diversity of tangerines in North Sumatra and support the government's efforts to increase quality tangerines. It can also be used as a basis for further development in agriculture and biotechnology.