Extraction and Antibacterial Activity of Atsiri Leaf Attarasa

Oct 13, 2025 by wbrum.org 81 views

Edible Film Antibacterial Activity from Pati Tapioca which is incorporated with Atsiri Leaf Attarasa [Litsea cubeba (Lour.) Press.]

Introduction

In recent years, the demand for environmentally friendly food packaging has been increasing, driven by consumer concerns about the impact of traditional packaging materials on the environment. One innovative approach in developing sustainable food packaging is the use of edible films made from starch tapioca. This study examines the antibacterial activity of edible films incorporated with Attarasa leaf essential oil (litsea cubeba) obtained through distillation using a stahl tool. The aim of this research is to evaluate the potential of Attarasa leaf essential oil as an antibacterial material and its application in edible film.

Background

Edible films are thin layers of edible materials that can be used as an alternative to traditional packaging materials. They are made from a variety of ingredients, including starch, protein, and lipids, and can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity. The use of edible films has several advantages, including reduced packaging waste, improved food safety, and enhanced consumer convenience.

Extraction and Antibacterial Activity of Atsiri Leaf Attarasa

Atsiri leaf essential oil has been tested by the diffusion method in order to evaluate its antibacterial activity. In this experiment, essential oils at a concentration of 10% (V/V) showed a transparent zone for Escherichia coli, Shigella, and Staphylococcus aureus bacteria with a diameter of 7.33 mm, 7.88 mm and 8.17 mm respectively. Furthermore, at a concentration of 15% (V/V), the transparent zone formed is 6.66 mm, 7.33 mm, and 10 mm for the same bacteria. These results indicate that Attarasa leaf essential oil has the potential as an effective antibacterial agent.

The antibacterial activity of Attarasa leaf essential oil can be attributed to the presence of various bioactive compounds, including terpenes, phenolics, and flavonoids. These compounds have been shown to have antimicrobial properties, which can help to prevent the growth of pathogenic bacteria and other microorganisms. The use of Attarasa leaf essential oil as an antibacterial agent has several advantages, including its natural origin, non-toxicity, and biodegradability.

Edible Film Making and Testing

Edible films made from starch tapioca (4% w/b) with glycerol (1% w/b) as a plastic and essential oil of Attarasa leaf (1.5% V/B) show antibacterial activity. The antibacterial activity test uses the diffusion method to show the transparent zone of the Escherichia coli (7.8 mm) and Shigella (7 mm) culture zones, although it does not show the inhibition zone for Staphylococcus aureus. This shows that edible films based on Pati Tapioca can be effective against several types of pathogenic bacteria.

The antibacterial activity of edible films made from starch tapioca and Attarasa leaf essential oil can be attributed to the presence of Attarasa leaf essential oil. The essential oil has been shown to have antimicrobial properties, which can help to prevent the growth of pathogenic bacteria and other microorganisms. The use of Attarasa leaf essential oil as an antibacterial agent has several advantages, including its natural origin, non-toxicity, and biodegradability.

Edible Film Application on Goldfish

Edible films made from tapioca starch and attraction leaf essential oil have been applied to goldfish packaging. The test was carried out by calculating the density of bacterial cell isolates using the Standard Plate Count (SPC) method on the first, third, fifth, and seventh day. The results show that the edible of the film can inhibit the growth of bacterial colonies in goldfish stored in the refrigerator. This shows the potential of edible films as an efficient and natural food preservation method.

The use of edible films as a food preservation method has several advantages, including improved food safety, reduced packaging waste, and enhanced consumer convenience. Edible films can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Conclusion

The use of edible films from Pati Tapioca which is incorporated with Atsiri Leaf Attarasa not only provides an environmentally friendly packaging solution, but also has an antibacterial potential that can inhibit the growth of pathogenic bacteria. This research opens the way for further development of safer and effective food packaging ingredients, while maintaining the quality of packaged food products. With the use of natural ingredients, it is hoped that this innovation can increase consumer confidence in the safety of the food they consume.

Future Directions

Further research is needed to fully understand the antibacterial activity of edible films made from starch tapioca and Attarasa leaf essential oil. This includes the study of the effects of different concentrations of Attarasa leaf essential oil on the antibacterial activity of edible films, as well as the study of the effects of different storage conditions on the antibacterial activity of edible films. Additionally, the development of new formulations of edible films with improved antibacterial activity is also an area of future research.

References

[1] S. S. S. S. (2020). Edible Film Antibacterial Activity from Pati Tapioca which is incorporated with Attarasa Leaf Essential Oil [Litsea cubeba (Lour.) Press.]. Journal of Food Science and Technology, 1-10.
[2] A. A. A. (2019). Antibacterial Activity of Attarasa Leaf Essential Oil against Escherichia coli, Shigella, and Staphylococcus aureus. Journal of Essential Oil Research, 1-8.
[3] B. B. B. (2018). Edible Films Made from Starch Tapioca and Attarasa Leaf Essential Oil: A Review. Journal of Food Engineering, 1-12.

Note: The references provided are fictional and for demonstration purposes only.
Edible Film Antibacterial Activity from Pati Tapioca which is incorporated with Atsiri Leaf Attarasa [Litsea cubeba (Lour.) Press.] - Q&A

Introduction

In our previous article, we discussed the antibacterial activity of edible films made from starch tapioca and Attarasa leaf essential oil. This innovative approach in developing environmentally friendly food packaging has several advantages, including improved food safety, reduced packaging waste, and enhanced consumer convenience. In this Q&A article, we will answer some of the most frequently asked questions about edible films and their antibacterial activity.

Q: What is the purpose of using edible films in food packaging?

A: Edible films are used in food packaging to provide a natural and biodegradable alternative to traditional packaging materials. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: How do edible films work as antibacterial agents?

A: Edible films made from starch tapioca and Attarasa leaf essential oil work as antibacterial agents by releasing the essential oil into the food environment. The essential oil has been shown to have antimicrobial properties, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: What are the benefits of using edible films in food packaging?

A: The benefits of using edible films in food packaging include improved food safety, reduced packaging waste, and enhanced consumer convenience. Edible films can also be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: Can edible films be used in a variety of food products?

A: Yes, edible films can be used in a variety of food products, including fresh produce, meat, dairy products, and baked goods. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: How do edible films compare to traditional packaging materials?

A: Edible films compare favorably to traditional packaging materials in several ways. They are natural and biodegradable, which reduces packaging waste and minimizes the environmental impact of food packaging. They also provide a natural and non-toxic alternative to traditional packaging materials, which can help to improve food safety and reduce the risk of foodborne illness.

Q: What are the future directions for edible films research?

A: Future directions for edible films research include the development of new formulations of edible films with improved antibacterial activity, the study of the effects of different concentrations of Attarasa leaf essential oil on the antibacterial activity of edible films, and the study of the effects of different storage conditions on the antibacterial activity of edible films.

Q: Can edible films be used in combination with other packaging materials?

A: Yes, edible films can be used in combination with other packaging materials, such as paper, plastic, and glass. This can help to improve the overall performance of the packaging material and provide a more effective barrier against pathogenic bacteria and other microorganisms.

Q: How do edible films affect the shelf life of food products?

A: Edible films can help to improve the shelf life of food products by providing a natural and non-toxic barrier against pathogenic bacteria and other microorganisms. This can help to reduce the risk of foodborne illness and improve the overall quality of the food product.

Q: Can edible films be used in food packaging for both fresh and processed foods?

A: Yes, edible films can be used in food packaging for both fresh and processed foods. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: What are the regulatory requirements for edible films in food packaging?

A: The regulatory requirements for edible films in food packaging vary depending on the country and region. In general, edible films must meet the same safety and quality standards as traditional packaging materials. They must also be labeled and packaged in accordance with applicable regulations.

Q: Can edible films be used in food packaging for both human and animal consumption?

A: Yes, edible films can be used in food packaging for both human and animal consumption. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: How do edible films affect the nutritional value of food products?

A: Edible films can help to improve the nutritional value of food products by providing a natural and non-toxic barrier against pathogenic bacteria and other microorganisms. This can help to reduce the risk of foodborne illness and improve the overall quality of the food product.

Q: Can edible films be used in food packaging for both hot and cold foods?

A: Yes, edible films can be used in food packaging for both hot and cold foods. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: What are the economic benefits of using edible films in food packaging?

A: The economic benefits of using edible films in food packaging include reduced packaging waste, improved food safety, and enhanced consumer convenience. Edible films can also help to reduce the risk of foodborne illness, which can result in significant economic losses for food manufacturers and retailers.

Q: Can edible films be used in food packaging for both organic and conventional foods?

A: Yes, edible films can be used in food packaging for both organic and conventional foods. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: How do edible films compare to other natural packaging materials?

A: Edible films compare favorably to other natural packaging materials in several ways. They are biodegradable and non-toxic, which reduces packaging waste and minimizes the environmental impact of food packaging. They also provide a natural and non-toxic alternative to traditional packaging materials, which can help to improve food safety and reduce the risk of foodborne illness.

Q: Can edible films be used in food packaging for both dry and wet foods?

A: Yes, edible films can be used in food packaging for both dry and wet foods. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: What are the environmental benefits of using edible films in food packaging?

A: The environmental benefits of using edible films in food packaging include reduced packaging waste, improved food safety, and enhanced consumer convenience. Edible films can also help to reduce the risk of foodborne illness, which can result in significant environmental impacts.

Q: Can edible films be used in food packaging for both individual and bulk packaging?

A: Yes, edible films can be used in food packaging for both individual and bulk packaging. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: How do edible films affect the shelf life of food products in different storage conditions?

A: Edible films can help to improve the shelf life of food products in different storage conditions by providing a natural and non-toxic barrier against pathogenic bacteria and other microorganisms. This can help to reduce the risk of foodborne illness and improve the overall quality of the food product.

Q: Can edible films be used in food packaging for both fresh and frozen foods?

A: Yes, edible films can be used in food packaging for both fresh and frozen foods. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: What are the regulatory requirements for edible films in food packaging in different countries?

Q: Can edible films be used in food packaging for both human and animal consumption in different countries?

A: Yes, edible films can be used in food packaging for both human and animal consumption in different countries. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: How do edible films affect the nutritional value of food products in different countries?

A: Edible films can help to improve the nutritional value of food products in different countries by providing a natural and non-toxic barrier against pathogenic bacteria and other microorganisms. This can help to reduce the risk of foodborne illness and improve the overall quality of the food product.

Q: Can edible films be used in food packaging for both hot and cold foods in different countries?

A: Yes, edible films can be used in food packaging for both hot and cold foods in different countries. They can be formulated to have specific properties, such as moisture barrier, gas barrier, and antibacterial activity, which can help to prevent the growth of pathogenic bacteria and other microorganisms.

Q: What are the economic benefits of using edible films in food packaging in different countries?

A: The economic benefits of using edible films in food packaging in different countries include reduced packaging waste, improved food safety, and enhanced consumer convenience. Edible films can also help to