Characterization of Simplicia and Phytochemical Screening

Oct 13, 2025 by wbrum.org 75 views

Introduction

Traditional medicine has been a cornerstone of healthcare for centuries, with many plants being used to treat various ailments. One such plant is Titanus (Leea aequata L.), which is commonly used in the Sukanalu Village, Barus Ginger District, Karo Regency, North Sumatra Province, to treat wounds, seizures, and tetanus. This study aims to characterize the isolation compounds from the ethyl acetate fraction of Titanus leaves using ultraviolet (UV) and infrared (IR) spectrophotometers.

Research Methodology

This study was conducted through several stages, including the characterization of simplicia, phytochemical screening, Titanus leaf simplicia powder extraction, Titanus leaf ethanol extract, and paper chromatography (KKT) with various mobile phases. The mobile phases used included Forestal, Baw (butanol: acetic acid: water), 15%acetic acid, 5%acetic acid, and 1%HCl. The stationary phase used was Whatmann No.1 paper, and the appearance of spots was shown by the use of AlCl3, FeCl3, and ammonia steam. The isolates obtained were then separated through the preparative KKT and were characterized by UV and IR spectrophotometers.

Characterization of Simplicia and Phytochemical Screening

The result of simplicia characterization showed that the water content reached 5.99%, water soluble levels 9.56%, extract content in ethanol 9.99%, total ash content 7.98%, and ash content not soluble in acid 1.15 %. The results of phytochemical screening in ethanol extract indicated the presence of several groups of active compounds, namely alkaloids, flavonoids, triterpenoids/steroids, tannins, saponins, and glycosides. In ethyl acetate fractions, there were flavonoids, tannins, saponins, and glycosides.

Isolation and Characterization of Compounds

From the isolation process, a pure isolate with a RF value of 0.66, which appeared in a bright yellow color when using AlCl3. Further characterization with UV spectrophotometer showed wavelengths at 356.2 Nm and 276.0 Nm. The results of the IR spectrophotometry analysis identified the presence of functional groups such as OH, C-H aliphatic, C = O Carbonyl, C-H. Aromatic, and C = Aromatic C. This finding indicated that the isolate was a flavonoid compound of the flavonol group.

Analysis and Discussion

Characterization of compounds from Titanus leaves is very important given the potential of traditional medicine that can be developed from this plant. Flavonoid compounds are known to have a variety of biological activities, such as antioxidant, anti-inflammatory, and antimicrobial, which has the potential to be used in modern medicine. This study shows that local plants, such as Titanus, store the treasures of biological wealth that need to be explored more deeply to find broader health benefits.

The existence of flavonoid compounds in ethyl acetate fractions also shows that the processing and separation of active compounds can be done by simple methods such as paper chromatography. The use of UV and IR spectrophotometry analysis methods is the key in determining the characteristics of isolated compounds.

Conclusion

Through this research, it is expected to trigger further interest in the exploration of traditional medicinal plants and explain the scientific basis of the use of these plants in the practice of local treatment. Such initiatives are not only important to preserve traditional knowledge, but also to support the development of natural drugs based on the bioactive components of nature.

Recommendations

Based on the findings of this study, the following recommendations are made:

Further research should be conducted to explore the potential of Titanus leaves in treating various diseases.
The use of UV and IR spectrophotometry analysis methods should be continued to determine the characteristics of isolated compounds.
The development of natural drugs based on the bioactive components of Titanus leaves should be supported.
The preservation of traditional knowledge and the promotion of the use of local plants in healthcare should be continued.

Limitations of the Study

This study has several limitations, including:

The study was conducted on a limited number of samples.
The study only focused on the characterization of compounds from Titanus leaves.
The study did not explore the potential of Titanus leaves in treating various diseases.

Future Directions

Future research should focus on the following areas:

The exploration of the potential of Titanus leaves in treating various diseases.
The development of natural drugs based on the bioactive components of Titanus leaves.
The preservation of traditional knowledge and the promotion of the use of local plants in healthcare.

Conclusion

In conclusion, this study has shown that Titanus leaves contain flavonoid compounds that have potential biological activities. The use of UV and IR spectrophotometry analysis methods has been shown to be effective in determining the characteristics of isolated compounds. Further research is needed to explore the potential of Titanus leaves in treating various diseases and to develop natural drugs based on the bioactive components of Titanus leaves.

Frequently Asked Questions

We have received many questions from readers regarding our study on the characterization of isolation compounds from ethyl acetate Titanus leaf fraction (Leea aequata L.) with UV and IR spectrophotometers. Below are some of the most frequently asked questions and our answers.

Q: What is Titanus (Leea aequata L.)?

A: Titanus (Leea aequata L.) is a plant that is commonly used in traditional medicine in the Sukanalu Village, Barus Ginger District, Karo Regency, North Sumatra Province. It is used to treat wounds, seizures, and tetanus.

Q: What is the purpose of this study?

A: The purpose of this study is to characterize the isolation compounds from the ethyl acetate fraction of Titanus leaves using UV and IR spectrophotometers.

Q: What are the methods used in this study?

A: The methods used in this study include the characterization of simplicia, phytochemical screening, Titanus leaf simplicia powder extraction, Titanus leaf ethanol extract, and paper chromatography (KKT) with various mobile phases.

Q: What are the results of the study?

A: The results of the study show that the water content of Titanus leaves is 5.99%, water soluble levels 9.56%, extract content in ethanol 9.99%, total ash content 7.98%, and ash content not soluble in acid 1.15 %. The results of phytochemical screening in ethanol extract indicate the presence of several groups of active compounds, namely alkaloids, flavonoids, triterpenoids/steroids, tannins, saponins, and glycosides.

Q: What are the implications of the study?

A: The study has several implications, including the potential of Titanus leaves in treating various diseases, the development of natural drugs based on the bioactive components of Titanus leaves, and the preservation of traditional knowledge and the promotion of the use of local plants in healthcare.

Q: What are the limitations of the study?

A: The study has several limitations, including the study was conducted on a limited number of samples, the study only focused on the characterization of compounds from Titanus leaves, and the study did not explore the potential of Titanus leaves in treating various diseases.

Q: What are the future directions of the study?

A: The future directions of the study include the exploration of the potential of Titanus leaves in treating various diseases, the development of natural drugs based on the bioactive components of Titanus leaves, and the preservation of traditional knowledge and the promotion of the use of local plants in healthcare.

Q: How can readers get involved in the study?

A: Readers can get involved in the study by contacting us directly and expressing their interest in participating in the study. We are always looking for new collaborators and researchers who can help us advance our understanding of the potential of Titanus leaves.

Q: What are the potential applications of the study?

A: The potential applications of the study include the development of new medicines based on the bioactive components of Titanus leaves, the preservation of traditional knowledge and the promotion of the use of local plants in healthcare, and the exploration of the potential of Titanus leaves in treating various diseases.

Q: What are the potential risks and benefits of the study?

A: The potential risks and benefits of the study include the potential for new medicines to be developed based on the bioactive components of Titanus leaves, the potential for traditional knowledge to be preserved and promoted, and the potential for new diseases to be treated with the use of Titanus leaves.

Conclusion

We hope that this Q&A article has provided readers with a better understanding of our study on the characterization of isolation compounds from ethyl acetate Titanus leaf fraction (Leea aequata L.) with UV and IR spectrophotometers. If you have any further questions or would like to get involved in the study, please do not hesitate to contact us.