Application of the MCR method

Oct 13, 2025 by wbrum.org 128 views

Determination of Simultaneous Levels of Acetic Hydrocortisone and Chloramphenicol in Cream Preparations with Ultraviolet Spectrophotometry Methods using the Mean Centering of Ratio Spectra (MCR)

Introduction

The use of combination drugs in the form of cream preparations has become increasingly popular in the pharmaceutical industry. Acetic hydrocortisone and chloramphenicol are two commonly used compounds in these preparations. However, determining the levels of these compounds simultaneously in cream preparations can be a challenging task. This study aims to determine the levels of acetic hydrocortisone and chloramphenicol in cream preparations using the ultraviolet spectrophotometry method, specifically through the Mean Centering of Ratio Spectra (MCR) approach.

The Importance of Determining Simultaneous Levels of Acetic Hydrocortisone and Chloramphenicol

Determining the levels of acetic hydrocortisone and chloramphenicol in cream preparations is crucial for ensuring the quality and efficacy of these products. The simultaneous determination of these compounds can provide valuable information on the stability and shelf life of the cream preparations. Moreover, it can also help in identifying any potential interactions between the two compounds, which can affect their therapeutic efficacy.

Research Methodology

This study took a chloramfecort cream sample, each containing 25 mg of acetate hydrocortisone and 20 mg of chloramphenicol. To measure the levels of the two substances, the MCR method was applied by measurement at a wavelength of 241 Nm for acetate hydrochortisone and 273 nm for chloramphenicol. Calculation of levels was done using the Matlab application that provides high accuracy in data analysis.

Characteristics of the MCR Method

The MCR method has several characteristics that make it an effective and reliable method for determining the levels of acetic hydrocortisone and chloramphenicol in cream preparations. These characteristics include:

Detection Limit (LOD): The LOD is the minimum concentration of a substance that can be detected using the MCR method. In this study, the LOD for acetate hydrocortisone was recorded at 0.635 μg/ml, while the LOD for chloramphenicol was recorded at 1.046 μg/ml.
Quantitation Limit (LoQ): The LoQ is the minimum concentration of a substance that can be quantitatively determined using the MCR method. In this study, the LoQ for acetate hydrocortisone was recorded at 2.1167 μg/ml, while the LoQ for chloramphenicol was recorded at 3.4871 μg/ml.
Accuracy: The accuracy of the MCR method was determined by comparing the measured levels of acetic hydrocortisone and chloramphenicol with the actual levels. The accuracy of the MCR method was found to be 100.7178% for acetate hydrocortisone and 99.4867% for chloramphenicol.
Precision: The precision of the MCR method was determined by calculating the relative standard deviation (RSD) of the measured levels of acetic hydrocortisone and chloramphenicol. The precision of the MCR method was found to be 0.6222% for acetate hydrocortisone and 0.7429% for chloramphenicol.
Selectivity: The selectivity of the MCR method was determined by analyzing the spectra of the cream preparations and identifying any potential interferences. The selectivity of the MCR method was found to be high, with no significant interferences detected.

Results and Discussion

The results of this study show that the MCR method is an effective and reliable method for determining the levels of acetic hydrocortisone and chloramphenicol in cream preparations. The measured levels of acetic hydrocortisone and chloramphenicol were found to be 101.5742 ± 2.3646% and 98.6768 ± 2.7674%, respectively. The accuracy and precision of the MCR method were found to be high, with accuracy values of 100.7178% and 99.4867% for acetate hydrocortisone and chloramphenicol, respectively. The detection limit and quantitation limit of the MCR method were found to be 0.635 μg/ml and 2.1167 μg/ml for acetate hydrocortisone, and 1.046 μg/ml and 3.4871 μg/ml for chloramphenicol, respectively.

Conclusion

Based on the results of this study, it can be concluded that the ultraviolet spectrophotometry method with the Mean Centering of Ratio Spectra (MCR) approach is a valid and effective method for determining the simultaneous levels of acetic hydrocortisone and chloramphenicol in cream preparations. The MCR method has several characteristics that make it an effective and reliable method, including high accuracy, precision, detection limit, and quantitation limit. These findings make an important contribution in the development of chemical analysis to pharmaceutical preparations, as well as strengthening trust in the use of pharmaceutical products that contain a combination of this drug.

Future Directions

This study provides a better understanding of the use of spectrophotometric techniques in chemical analysis and encourages further research in the fields of pharmacy and chemical analysis. Future studies can focus on applying the MCR method to other pharmaceutical preparations and investigating the potential applications of this method in other fields.

Limitations

This study has several limitations that should be addressed in future studies. The MCR method is sensitive to the wavelength of measurement, and the accuracy and precision of the method may be affected by the choice of wavelength. Additionally, the MCR method may not be suitable for all types of pharmaceutical preparations, and further studies are needed to investigate the applicability of this method to other types of preparations.

Recommendations

Based on the results of this study, the following recommendations can be made:

Use of the MCR method: The MCR method is a valid and effective method for determining the simultaneous levels of acetic hydrocortisone and chloramphenicol in cream preparations.
Application of the MCR method: The MCR method can be applied to other pharmaceutical preparations to determine the levels of other compounds.
Investigation of the MCR method: Further studies are needed to investigate the potential applications of the MCR method in other fields and to address the limitations of this method.
Frequently Asked Questions (FAQs) about Determining Simultaneous Levels of Acetic Hydrocortisone and Chloramphenicol in Cream Preparations using the Mean Centering of Ratio Spectra (MCR) Method

Q: What is the Mean Centering of Ratio Spectra (MCR) method?

A: The MCR method is a spectrophotometric technique used to determine the levels of multiple compounds in a sample. It involves measuring the ratio of the absorbance of each compound at a specific wavelength and then applying a mathematical algorithm to calculate the concentrations of the compounds.

Q: What are the advantages of using the MCR method?

A: The MCR method has several advantages, including high accuracy, precision, detection limit, and quantitation limit. It is also a relatively simple and cost-effective method compared to other spectrophotometric techniques.

Q: What are the limitations of the MCR method?

A: The MCR method has several limitations, including sensitivity to the wavelength of measurement and potential interferences from other compounds in the sample. Additionally, the method may not be suitable for all types of pharmaceutical preparations.

Q: How does the MCR method work?

A: The MCR method involves measuring the absorbance of each compound at a specific wavelength and then applying a mathematical algorithm to calculate the concentrations of the compounds. The algorithm takes into account the ratio of the absorbance of each compound and the absorbance of the sample at the specific wavelength.

Q: What are the applications of the MCR method?

A: The MCR method has several applications in the pharmaceutical industry, including the determination of the levels of multiple compounds in cream preparations, tablets, and capsules. It can also be used to monitor the stability and shelf life of pharmaceutical products.

Q: What are the benefits of using the MCR method in the pharmaceutical industry?

A: The MCR method has several benefits in the pharmaceutical industry, including improved accuracy and precision, reduced costs, and increased efficiency. It can also help to ensure the quality and efficacy of pharmaceutical products.

Q: How can the MCR method be used to improve the quality and efficacy of pharmaceutical products?

A: The MCR method can be used to improve the quality and efficacy of pharmaceutical products by ensuring that the levels of multiple compounds are within the specified limits. It can also help to identify potential interactions between compounds and ensure that the product is stable and shelf life is extended.

Q: What are the future directions for the MCR method?

A: The MCR method has several future directions, including the application of the method to other types of pharmaceutical preparations, the development of new algorithms for calculating the concentrations of compounds, and the use of the method to monitor the stability and shelf life of pharmaceutical products.

Q: How can the MCR method be used to monitor the stability and shelf life of pharmaceutical products?

A: The MCR method can be used to monitor the stability and shelf life of pharmaceutical products by measuring the levels of multiple compounds over time. This can help to identify any changes in the product that may affect its quality and efficacy.

Q: What are the potential applications of the MCR method in other fields?

A: The MCR method has several potential applications in other fields, including the determination of the levels of multiple compounds in environmental samples, food samples, and biological samples. It can also be used to monitor the stability and shelf life of products in other industries.

Q: How can the MCR method be used to determine the levels of multiple compounds in environmental samples?

A: The MCR method can be used to determine the levels of multiple compounds in environmental samples by measuring the absorbance of each compound at a specific wavelength and then applying a mathematical algorithm to calculate the concentrations of the compounds.

Q: What are the benefits of using the MCR method in environmental monitoring?

A: The MCR method has several benefits in environmental monitoring, including improved accuracy and precision, reduced costs, and increased efficiency. It can also help to ensure the quality and efficacy of environmental monitoring programs.

Q: How can the MCR method be used to determine the levels of multiple compounds in food samples?

A: The MCR method can be used to determine the levels of multiple compounds in food samples by measuring the absorbance of each compound at a specific wavelength and then applying a mathematical algorithm to calculate the concentrations of the compounds.

Q: What are the benefits of using the MCR method in food analysis?

A: The MCR method has several benefits in food analysis, including improved accuracy and precision, reduced costs, and increased efficiency. It can also help to ensure the quality and efficacy of food products.

Q: How can the MCR method be used to determine the levels of multiple compounds in biological samples?

A: The MCR method can be used to determine the levels of multiple compounds in biological samples by measuring the absorbance of each compound at a specific wavelength and then applying a mathematical algorithm to calculate the concentrations of the compounds.

Q: What are the benefits of using the MCR method in biological analysis?

A: The MCR method has several benefits in biological analysis, including improved accuracy and precision, reduced costs, and increased efficiency. It can also help to ensure the quality and efficacy of biological products.