Analysis of Treatment Interactions

Oct 13, 2025 by wbrum.org 112 views

Optimizing Centelloside Production in Gotu Kola (Centella Asiatica) through Phosphorus and Methyl Physical Administration

Introduction

Gotu kola (Centella asiatica) is a wild plant that has been widely used for its medicinal properties, containing bioactive compounds such as centelloside. However, excessive use of gotu kola from nature without structured cultivation can threaten its sustainability. Therefore, it is essential to develop efficient and sustainable cultivation methods to optimize centelloside production. This study aimed to investigate the effect of phosphorus and methyl physical administration on centelloside production in gotu kola, as well as determine the optimal harvest age.

Materials and Methods

This study involved 108 experimental plots with an area of 1 square meter per plot. The researchers carried out a combination of phosphorus treatment (0, 18, 36, and 54 kg P2O5/ha), methyl physical (0, 100, and 200 μm), and harvest age (56, 70, and 84 days after planting). The study was conducted to evaluate the effect of these treatments on centelloside production, vegetative growth, and other plant components.

Results

The results showed that the administration of phosphorus had a positive effect on the vegetative growth of gotu kola, such as leaf stalks, the number of leaves, leaf area, and the number of tendrils. The administration of 36 kg P2O5/ha phosphorus produced the most optimal vegetative growth. However, the administration of 54 kg P2O5/ha phosphorus produced higher leaf asiaticoside content, while a dose of 18 kg P2O5/ha produced higher leaf-based acid content.

Giving methyl physical 100 μm and 200 μm had an impact on the decrease in phosphorus content in plant tissue. Harvest age turned out to have a significant effect on the acidic content of leaf and Madekasoside root. Harvest at the age of 84 days after planting produced the highest production of Madekasosida leaf and roots.

Analysis of Treatment Interactions

Analysis of treatment interactions showed that a combination without methyl physical with harvest age 84 days after planting produced the highest biomass production, both for wet wet and petiol weight or wet root and tendrils. The combination of methyl physical giving of 200 μm with harvest age 84 days after planting produced the highest asiaticoside content in roots and tendrils.

The interaction between the dose of phosphorus and the harvest age showed that the combination of harvest age 84 days after planting and fertilizing 54 kg P2O5/ha phosphorus produced the highest root and tendril madekasoside content.

Interaction between Methyl Physical Concentrations and Phosphorus Doses

The results also showed an interaction between methyl physical concentrations and phosphorus doses, especially in production components such as wet weight and dry weight per plot. The combination of methyl physical administration of 100 μm and fertilizing 18 kg P2O5/ha phosphorus produced the highest production for wet wet and petiol weight, as well as dry root and tendrils.

Conclusion

Overall, this study shows that the right combination of treatment, namely the administration of methyl physical 100 μm and 18 kg P2O5/ha phosphorus with a harvest age of 84 days after planting, is an optimal formula for increasing centelloside production in gotu kola. This study provides important information for the development of more efficient and sustainable gotu kola cultivation. By paying attention to the results of this study, it is expected to increase the production of kola centerside and encourage optimal use for various purposes, both in the field of pharmacy and industry.

Implications for Sustainable Gotu Kola Cultivation

The results of this study have significant implications for sustainable gotu kola cultivation. The optimal combination of phosphorus and methyl physical administration, as well as the determination of proper harvest age, can help to increase centelloside production and reduce the pressure on wild gotu kola populations. This can contribute to the conservation of this valuable plant species and ensure its continued availability for medicinal and industrial purposes.

Future Research Directions

Future research should focus on scaling up the results of this study to larger plots and evaluating the economic viability of the optimal formula for centelloside production. Additionally, further research is needed to investigate the effects of other factors, such as soil type and climate, on centelloside production in gotu kola. This will help to develop more efficient and sustainable cultivation methods for this valuable plant species.

References

This study was conducted to optimize the production of centelloside in gotu kola through the treatment of phosphorus and methyl physical, as well as the determination of proper harvest age. The results showed that the administration of phosphorus had a positive effect on the vegetative growth of gotu kola, while the administration of methyl physical had an impact on the decrease in phosphorus content in plant tissue. Harvest age turned out to have a significant effect on the acidic content of leaf and Madekasoside root.

The analysis of treatment interactions showed that a combination without methyl physical with harvest age 84 days after planting produced the highest biomass production, while the combination of methyl physical giving of 200 μm with harvest age 84 days after planting produced the highest asiaticoside content in roots and tendrils. The interaction between the dose of phosphorus and the harvest age showed that the combination of harvest age 84 days after planting and fertilizing 54 kg P2O5/ha phosphorus produced the highest root and tendril madekasoside content.

Q: What is centelloside, and why is it important?

A: Centelloside is a bioactive compound found in gotu kola (Centella asiatica), a wild plant that has been widely used for its medicinal properties. Centelloside has been shown to have various health benefits, including anti-inflammatory, antioxidant, and anti-cancer properties. Therefore, optimizing centelloside production is crucial for the development of more efficient and sustainable gotu kola cultivation.

Q: What are the key factors that affect centelloside production in gotu kola?

A: The key factors that affect centelloside production in gotu kola include phosphorus and methyl physical administration, harvest age, and soil type. This study investigated the effect of phosphorus and methyl physical administration on centelloside production, as well as determined the optimal harvest age.

Q: What is the optimal combination of phosphorus and methyl physical administration for centelloside production?

A: The results of this study showed that the optimal combination of phosphorus and methyl physical administration for centelloside production is the administration of methyl physical 100 μm and 18 kg P2O5/ha phosphorus with a harvest age of 84 days after planting.

Q: How does harvest age affect centelloside production in gotu kola?

A: Harvest age turned out to have a significant effect on the acidic content of leaf and Madekasoside root. Harvest at the age of 84 days after planting produced the highest production of Madekasosida leaf and roots.

Q: What are the implications of this study for sustainable gotu kola cultivation?

A: The results of this study have significant implications for sustainable gotu kola cultivation. The optimal combination of phosphorus and methyl physical administration, as well as the determination of proper harvest age, can help to increase centelloside production and reduce the pressure on wild gotu kola populations. This can contribute to the conservation of this valuable plant species and ensure its continued availability for medicinal and industrial purposes.

Q: What are the future research directions for optimizing centelloside production in gotu kola?

A: Future research should focus on scaling up the results of this study to larger plots and evaluating the economic viability of the optimal formula for centelloside production. Additionally, further research is needed to investigate the effects of other factors, such as soil type and climate, on centelloside production in gotu kola.

Q: How can the results of this study be applied in practice?

A: The results of this study can be applied in practice by farmers and researchers who are interested in optimizing centelloside production in gotu kola. By following the optimal combination of phosphorus and methyl physical administration, as well as the determination of proper harvest age, farmers can increase centelloside production and reduce the pressure on wild gotu kola populations.

Q: What are the potential applications of centelloside in the pharmaceutical and industrial sectors?

A: Centelloside has been shown to have various health benefits, including anti-inflammatory, antioxidant, and anti-cancer properties. Therefore, it has potential applications in the pharmaceutical sector for the development of new medicines. Additionally, centelloside can be used as a natural ingredient in the production of cosmetics and food products.

Q: How can the results of this study contribute to the conservation of gotu kola?

A: The results of this study can contribute to the conservation of gotu kola by providing a more efficient and sustainable method for cultivating this valuable plant species. By reducing the pressure on wild gotu kola populations, farmers can help to conserve this species and ensure its continued availability for medicinal and industrial purposes.